Our new review, led by Helna, examines how extracellular vesicles (EVs) interact with key biological barriers, including cartilage, the blood–brain barrier, the gastrointestinal tract, the eye, and the skin, and the effects on pharmacokinetics and tissue transport. We also present engineering design principles to enhance EV targeting and overcome these barriers for more effective therapeutics. The paper is now available online, read it [HERE]!